Nucleoredoxin Downregulation Reduces β-Catenin Levels and Shifts Hematopoietic Differentiation towards Myeloid Lineage In Vitro

The importance of dissecting signaling pathways governing cell differentiation is based on their relevance not only for understanding basic biological phenomena but also better comprehending the underlying mechanisms pathologic alterations such as cancer. A paradigm processes hematopoiesis, where a single stem gives rise to multiple, fully differentiated, lineages. Nucleoredoxin (Nrx), member thioredoxin family, an important redox-sensitive modulator Wnt/β-catenin signaling, key pathway control hematopoiesis. In this work, Nrx mouse hematopoietic progenitor cells has been analyzed in vitro. silencing leads dramatic reduction size Lin− and LSK populations. Moreover, there remarkable decrease CD3+ enhancement percentage CD11b+Gr1− myeloid cells. This bias would agree with inhibition pathway. Interestingly, β-catenin at protein level was observed upon silencing. Our results strongly support differentiation, which could be mediated by regulation